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As shown in Figure 1, in the presence of MEDI-565, but not Cont BiTE, tumour cells were surrounded by T cells, detached from the bottom of the wells, and showed apoptotic changes in cell morphology.
Metastatic tumour explants cultured with MEDI-565, but not Cont BiTE, were surrounded by T cells, detached from the bottom of the well, and were gradually killed (Supplementary Figure 1).
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We also performed western blot analysis using an anti-GFP antibody to detect the expression of the zTERT-GFP protein, and its reduction by zTERT-MO1 but not Cont-MO1 (Figure 1A, f, g).
Interestingly, MSC-DCs, but not cont-DCs, were able to induce Tregs efficiently when cocultured with naive T cells (Fig. 5B).
Interestingly, T cells proliferated in MEDI-565 containing co-cultures, but not in Cont BiTE containing co-culture (3.31±0.49 × 10 vs 0.72±0.11 × 10 cells per well), possibly because of activation as evidenced by upregulation of CD69 and CD25 observed only in MEDI-565 cultures.
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