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The genes predicted by XRAY analysis to be significantly differentially expressed but not alternatively spliced (DE p<0.001 and AS p>0.001), significantly alternatively spliced and constitutively expressed (AS p<0.001 and DE p>0.001) and both significantly differentially expressed and alternatively spliced (DE p<0.001 and AS p<0.001) were used for Ingenuity Pathway Analysis.
In sum, we demonstrate that during T and B lymphocyte activation there are three classes of genes changing as a function of time: alternatively spliced and differentially expressed (AS+DE), constitutively expressed and alternatively spliced (AS+CE) and differentially expressed but not alternatively spliced (DE).
0 indicates that the exon was absent (one band at the smallest expected size); 1 indicates the exon was present, but not alternatively spliced (one band seen at the largest expected size); 2 indicates that the exon was present and alternatively spliced (2 bands observed).
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These arrays were designed to identify differentially expressed genes, but not their alternatively spliced transcript forms.
Furthermore, pyrimidine +3 was also seen adjacent to all β-dystrobrevin exon 13 sequences, which are also alternatively spliced[ 9], but not γ-dystrobrevin exon 13, which is not alternatively spliced (this work; [see Additional File 2]).
Objective: To investigate whether or not alternatively spliced variants of the FSH receptor gene occur in human testis and whether the presence of the splicing variants is associated with spermatogenic defects and serum FSH concentration in infertile men.
Still it could be argued that more number of accessible stations is preferable from the users' perspective; nevertheless, users commonly use a single station and do not alternatively use or switch between stations.
Such children may be indiscriminately sociable and approach all adults, whether familiar or not; alternatively, they may be emotionally withdrawn and fail to seek comfort from anyone.
TPs were grouped as Non-AS-TPs if transcripts were not alternatively spliced.
SmDia is a single copy gene without introns encoding a transcript of 3.5 kb, which occurs most abundantly in males, and which is probably not alternatively spliced.
Using the above described methods, all of them are shown to be not alternatively spliced.
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