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10.7554/eLife.10874.017 Figure 8. islet1 is required for initiation of Runx1, but not Cbfb expression.
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(A – D ) In situ hybridization analysis of expression of Runx1 (A and B ) and Cbfb (C and D ) in control and Isl1 CKO DRGs at E12.5 shows that Islet1 deficiency abolishes expression of Runx1 but not Cbfb at an early age.
On the other hand, Islet1, a LIM-homeodomain transcription factor, controls transcriptional initiation of Runx1 but not Cbfb.
BBR enhanced the PKD1 catalytic activity, but not its expression.
Topotecan affects allelic expression of Ube3a and Ube3a-ATS but not expression of nearby genes.
Familiarity facilitated identity but not expression matching.
(A ) Real-time PCR analysis of Cbfb expression in control and Ngf -/-Bax -/- DRGs at E14.5 and P0 reveals early onset of NGF dependence for Cbfb expression.
Generation of the Cbfb Flag allele and more detailed characterization of the temporal and spatial patterns of Cbfb expression.
(C and D ) In situ hybridization analysis of Cbfb expression in control and Ngf -/-Bax -/- DRGs at E16.5 shows more pronounced Cbfb mRNA deficit in Ngf -/-Bax -/- DRGs.
Cbfb is also sensitive to the dose of NGF as exogenous NGF administered to wildtype neonates via intraperitoneal injection further potentiated Cbfb expression.
10.7554/eLife.10874.015 Figure 7. NGF promotes Cbfb expression through the ERK/MAPK signaling pathway.
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