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We found moderate to strong nuclear Stat6 expression in all SFTs but no expression in the other tumors.
Sequencing of wild-type and white cDNAs from embryos revealed splice variants in each background yet white cDNAs consistently lacked exon 2. RT-PCR using primers to detect coding sequence for the mature Edn3 peptide revealed initially low but increasing levels of transcript in wild-type embryos beginning during stages of neural crest migration, but no expression in white mutants (Fig. 1B,C).
This region shows good embryonic expression but no expression in adult muscles.
They show high expression levels only in pupae (P18) and adults (A4), respectively, but no expression in other stages.
Also, pups from mothers exposed to dox showed cartilage-specific expression, but no expression in liver, brain or skin.
Another particular aspect observed in our cases was Mena positivity in lymphoblasts and endothelial cells but no expression in lymphoma cells [ 22].
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It is strongly expressed in the follicle cells but shows no expression in the female germline.
Those genes which were expressed in the BM of patients but had no expression in the BM from healthy volunteers were considered to be derived from cancer cells lodged in the BM.
For BphQTLch4, one resistant IL-BPH carried a functional sesquiterpene synthase (STPS) gene located on the long arm of chromosome 4. STPS was up-regulated by BPH infestation in the IL-BPH but with no expression in infested KDML, which carries a large mutation in its promoter region (Kamolsukyunyong et al. 2013).
In contrast, staining for cleaved caspase 3, a marker of apoptosis, revealed diffuse staining throughout SmoA1 +; Pten +/+ medulloblastomas, but virtually no expression in SmoA1 +; Pten +/− tumors (Figure 5B).
We also profiled DRAM-3 expression in human tumor cell lines, which again showed expression in some lines, but virtually no expression in others.
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