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Gastric macrophages were identified as cells expressing CD11b and major histocompatibility complex class II (MHC-II), but lacking expression of Gr1 (neutrophil marker), CD103 (expressed by a subset of dendritic cells (DCs)) and sialic acid-binding immunoglobulin-like lectin (Siglec-F, eosinophil marker) (Fig. 1B).
SYF cells, deficient for tyrosine kinases Src, Yes, and Fyn, and Src++ cells, a control expressing endogenous wild-type Src but lacking expression of Yes and Fyn, were mouse fibroblasts obtained from the American Type Culture Collection (ATCC).
CTCs were defined as nucleated, epithelial cell adhesion molecule (EpCAM)–positive cells, expressing cytoplasmic cytokeratins 8, 18 and 19, but lacking expression of the common leukocyte antigen CD45.
Breast cancer stem cells have been isolated from human breast tumors or breast cancer-derived pleural effusions using flow cytometry to find subpopulations of cells with a specific pattern of cell surface markers (CD44+, CD24−/low, ESA+ (epithelial specific antigen)) but lacking expression of specific lineage markers (Lin−) [10].
In all experiments, littermates carrying the respective loxP-flanked alleles but lacking expression of Cre recombinase were used as wild-type (WT) controls.
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SC/CS tumor cells showed both expansive and invasive growth patterns, an exclusive and heterogeneous expression of CK8 and CK14, and predominantly expressed vimentin, but lacked expression of E-cadherin and SMA (Table 1; supplementary material Table S1).
BMMSCs and WJMSCs express low levels of MHC class I but lack expression of HLA-DR and co-stimulatory ligands, CD80 and CD86.
ACs showed membranous expression of E-cadherin and displayed a mixed but exclusive expression pattern of CK8 and CK14, but lacked expression of vimentin and SMA (Fig. 5A; supplementary material Table S1).
Breast cancer cells lacking expression of CXCL12 but exhibiting CXCR4 can metastasize to target organs that secrete CXCL12 [47].
Sertoli cell-only tubules were detected in testes from mice lacking expression of ERCC1 or MSH2 but not p53.
Cao, X. et al. Defective lymphoid development in mice lacking expression of the common cytokine receptor γ chain.
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