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PBP2′ does not wholly lack affinity for β-lactams, but its affinity for available analogues is very weak.

With in vitro-binding experiments, we find that the GTG3A mutation indeed has a lower affinity for GRE sequences compared with WT GR, but its affinity for nGREs remains relatively unchanged (Supplementary Figure 1b-c).

Previously reported propellane derivative KNT-42 preferred the κ receptor and functioned as a message part in the message-address concept, but its affinity for the κ receptor was not high.

In contrast, the affinity of imiloxan for the α1-adrenoceptor subtypes is very low (pKi < 4; which excludes its interaction with these receptors), but its affinity for the α2B (pKi: 7.3) and α2C (pKi: 6.0) subtypes (Table 1) leaves very little room for in vivo selectivity, particularly at the doses used (Fig. 6b).

This model assumes that the affinity for DnaA-ATP binding to its own promoter can change with growth rate but its affinity for the origin does not.

Bevacizumab is cross-reactive with rabbit VEGF, but its affinity for rabbit VEGF is one-eighth that for human VEGF [ 22].

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The engineered BMP-2 variant L51P is deficient in BMP receptor type I binding, but maintains its affinity for BMP receptor type II and BMP antagonists including Noggin, Chordin and Gremlin.

The present results demonstrating that phosphorylation of PAGE4 at T51 is critical for its ability to potentiate transactivation by c-Jun but weaken its affinity for c-Jun appear to be counterintuitive.

The ubiquitous gas, carbon monoxide (CO), is of substantial biological importance, but apart from its affinity for reduced transition metals, particularly heme-iron, it is surprisingly nonreactive-as is the ferrous-carbonyl-in living systems.

The designed two carboxyl groups not only greatly improved the water solubility of this chemosensor but also increased its affinity for Zn2+.

The MBD is unusual among site-specific DNA binding proteins in that (i) specificity is not conferred by the enhanced affinity for the specific site but rather by suppression of its affinity for generic DNA, (ii) its specific binding to mCpG is highly electrostatic, and (iii) it takes up as well as displaces monovalent cations upon DNA binding.

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