We identified nine chromosomal segments flanked by syntenic sequences but were in reverse orientations between the H99 and JEC21 genomes.
No similarity to r1 is detected in region D. Region E (69,850 bp) again aligns well with r1, but in reverse orientation, indicating a genomic inversion.
In type I TA systems, toxin gene expression is regulated by an antisense RNA (RNA-antitoxin) transcribed from a gene adjacent to the toxin gene but in reverse orientation.
The Cτ sequences in the IGH-A are as follows: starting from the 5' end; 1) CτA-1, partial (most of exon 4 only), 2) CτA-2, partial (5' start to approximately 40 base pairs into exon 3), 3) CτA-3, complete but has a frameshift, 4) CτA-4, complete but in reverse orientation, and 5) CτA-5, complete.
It is flanked by perfect direct repeats (one orientated in reverse orientation) and is integrated between two asn tRNAs, of which one in the reverse orientation.
However, this region is flanked by perfect direct repeats (one orientated in reverse orientation), and it is integrated between two asn tRNAs, of which one is in the reverse orientation.
The genomic region coding for the D7 proteins in supercontig1.204 shows three short D7 genes followed by two genes coding for long forms; however, while in Anopheles the frame orientation of the short and long forms is the same (but the short and long forms are in reverse orientation to each other), in Ædes there is no consistent orientation.
In these constructs, named CNS K LR) and CNS L LR), the CNS not only is located far away from the promoter, but it can also be considered that is in reverse orientation with respect to the SV40 promoter.
For gene disruption, a knockout construct with the Bsr cassette in reverse orientation was amplified using the carB sense and carB antisense primers and transfected into DD AX2 and AX3 cells by electroporation.
A short fragment of lacZ was cloned into the pBAD expression vector, in reverse orientation, using the EcoRI and PstI restriction sites.
