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Moreover, when Leeuwenhoek observed the swarming of his animalcules but failed to observe their division, he could only reinforce the idea that they arose spontaneously.
We also performed the GMSA assays with the HsmtSSB proteins at 20 and 100 mM NaCl, but failed to observe any differences in binding affinities (data not shown).
For this purpose we used B2 cells lacking TACI and BCMA, but failed to observe a role for these molecules in CD40-dependent IgA production.
Another study which analyzed SNPs in the RBP4 for association with type 2 diabetes identified a common haplotype associated with type 2 diabetes but failed to observe any single SNP association [12].
We studied whether or not the hemocytes in the hemolymph actually flows through the tubular embryonic heart using the live imaging system, but failed to observe any hemocytes flowing through the embryonic heart during embryonic stage (T.N.S, unpublished result).
We stained for MMP9 in HUVECs in the gradient chamber but failed to observe any consistent change in intensity.
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If so, however, we might have expected to observe differences in the pattern of response within the sagittal plane, but we failed to observe any such differences.
But we failed to observe a major efficacy after giving intravesical IFN-α2b.
But we failed to observe a significant activation of autophagy in any tested concentrations indicated in Figure 3c.
IL-6 was added to tumour cells in 3D cultures but we failed to observe any stimulation of tumour cell invasion (Fig. 4E).
To gain further insight into Abd-A expression in CCAP neurons we stained for Abd-A in Ubx mutants, but we failed to observe any expression in the CCAP neurons of first instar larvae (data not shown).
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