Sentence examples for but failed to interact from inspiring English sources

As expected, NIXΔBH3 was able to localize to mitochondria (Figure 3B), but failed to interact with Mieap-full (Figure 3F and 3G).

Wild-type myc-LITAF expressed in HEK-293T cells specifically bound to GST-fusion proteins of full-length Itch and the isolated WW domains of Itch, but failed to interact with GST alone or the GST-fused PRD domain of Itch.

Endogenous ECD also immunoprecipitated WT PIH1D1 but failed to interact with PIH1D1 K64A.

ORF57-6xHis protein was precipitated by GST-Aly, but failed to interact with GST-Hpr1 or GST alone.

As expected, PYM-FLAG precipitated ORF57, Magoh and eIF4G, whereas PYMΔN19-54-FLAG PYMΔN19-54-FLAG PYMΔN19-54-FLAGted ORF57, but fanded to interact with Magoh and eIF4G.

Last, by using a reconstitution assay of synthesized proteins, we showed that the D326V missense pRb bound to E2F1 but failed to interact with E2F2/3.

WDR62 associates directly with the MKK7β1 isoform independently of JNK binding, but fails to interact with MKK7α1.

Moreover, shorter RNA substrates bind more weakly than the longer RNAs to the Lsm1-7-Pat1 complex and human Pat1C interacts with U30 but fails to interact with U20 and U15.

Accordingly, a mutant version of Srs2 that retains wild type levels of ATPase and helicase activities, but fails to interact with Rad51 is compromised for its anti-recombinase function [30].

As in MEFs, the MUS81 loss-of-interaction mutants failed to interact with SLX4 in co-immunoprecipitation experiments (Fig. 2C).

Importantly, the association of Dvl-1 with Axin 1 and GSK-3 β was disrupted upon the expression of wild-type but not the mutated CXXC4 that failed to interact with Dvl-1.