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One of the transformation-defective mutants localized to the cell nucleus but failed to bind DNA.
Both immobilized mimodyes successfully bound P. fluorescens GaDH but failed to bind the enzyme from rabbit muscle.
Single chain fragment variables were prepared from presently available decavalent monoclonal anti-slan IgM antibodies but failed to bind to slanDCs.
The 80 kDa fragment bound only with untreated erythrocytes and trypsin treated erythrocytes but failed to bind with neuraminidase treated and chymotrypsin treated erythrocytes (Figures 3A and S6B).
Four mAbs (HX44, HGP105, HGW7, HGY38) reacted with wt and CD4bs YU2 mutants but failed to bind the CD4i mutant, and therefore were assigned to the CD4i cluster.
Consistent with previous reports, we found that the recombinant rPfRH430 protein bound neuraminidase treated erythrocytes but failed to bind with trypsin or chymotrypsin treated erythrocytes (Figure 3E).
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This chimera copurifies with FAD and displays demethylase activity, but fails to bind the partner protein corepressor of the RE1-silencing transcription factor (CoREST).
Enhanced TRP channel activity and migration in Dbn SMCs were normalized to WT levels by rescue expression of not only WT Drebrin but also a mutant Drebrin isoform that binds actin but fails to bind Homer.Drebrin reduces SMC activation through its interaction with the actin cytoskeleton but independently of its interaction with Homer scaffolds.
These results are in agreement with the results for RVF-VLPs generated with GnK48 (Fig. 4A), in that GnK48 can bind N but fails to bind RdRp.
Proteins carrying these mutations still bind to microtubules (BA et al., manuscript in preparation), but fail to bind the α4 protein [7] (BA et al., manuscript in preparation).
Mutants of cyclin D1 that fail to accumulate in the nucleus but bind cdk4, or that accumulate in the nucleus but fail to bind cdk4 were both unable to support tumor development.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com