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The real-time PCR genotyping method described herein showed high accuracy at the subtype level but failed to amplify 3% of the samples.
Moreover, both the 1st and 2nd 3'RACE pri-miR-433 stopped at the predicted TTS but failed to amplify the full length pre-miR-127.
Since herpesvirus genomes are linear when packaged into virions and only circularize in the nucleus [38], this result suggested that genomes still reached the nucleus without ORF75c, but failed to amplify the genome load by lytic replication.
PCR reactions undertaken with the remaining 8 Peruvian samples, which were negative for pfhrp2, resulted in amplification of the expected product for the downstream gene but failed to amplify the gene upstream of pfhrp2, in a pattern resembling that observed with D10.
In parallel experiments, IL-6 additivelytively with Flt-3L to increase cell numbers and IL-6-deficient bone marrow cultures (compared to wildtype controls) but failed to amplify in response to Flt-3L and PTH, suggesting that IL-6 mediated the PTH effect.
These 18 Mycobacterium spp. isolates were acid-fast on microscopic smear examination but failed to amplify the expected 372 bp product in the PCR protocol used to identify M. tuberculosis complex bacteria (data not shown).
Similar(52)
The nested amplification described by Martin and Rygiewicz [42] was effective at eliminating plant sequences, but also failed to amplify sequences several fungal taxa that were detected using the ENDOITS primers (Table 3 and Figure 1).
In addition, a third hypermutator isolate (3c) was obtained from the same patient in 2008, which possessed an unaltered mutL sequence but which failed to amplify a region of the mutS gene.
Primers VRNBPR2 and VRNBPF2 were 72 bp apart in vrn-B1 in Lebsock, but they failed to amplify the expected PCR product in PI 94749.
19 of these proteins were determined to be GRP candidates, but 10 failed to amplify and/or sequence in Type B individuals; the remaining nine were selected for further study.
We did amplify transcripts spanning from PPARG exon A1 to TSEN2 exon 2 but we failed to amplify any RT-PCR product spanning from PPARG exon B to TSEN2 exon 2. The experiment was repeated several times with different primer sets, using primers that allowed the amplification of the individual gene transcripts, and the results were always the same.
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but failed to increase
but failed to intensify
but failed to pass
but failed to attract
but failed to hold
but failed to do
but failed to rediscover
but failed to retain
but failed to say
but failed to achieve
but chose to amplify
but failed to present
but failed to persuade
but failed to throw
but failed to secure
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