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Using site-directed mutagenesis approach to substitute important residues used in catalysis in bacterial enzymes, which are conserved in ScASNase1, we show that substitutions abrogated the enzyme activity but do not exert significant effects on the secondary structure of the enzyme.
PLC and MLCK signaling also play roles in adhesion, but do not exert NRG1α-specific effects.
In the present cell culture system, the stable expression of α-synuclein or the A53T mutated form leads to the accumulation of small punctate aggregates throughout the cytoplasm, which are more abundant in cells expressing the A53T mutation, but do not exert cytotoxic effects per se.
In contrast, other states may require localities to submit water plans and may require permits for withdrawals in designated areas, but do not exert control statewide.
The other two factors, diagnosis and therapy, result as a consequence of the aforementioned three primary factors, but do not exert any major influence themselves.
Therefore, 1% TBSA burn injuries in the hindpaw can cause spinal cord VHMN apoptosis but do not exert any systemic effects inducing inflammatory cytokine production.
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The provision of suspended pieces of hard wood modified the pigs' behaviors but did not exert relevant effects on fecal corticosteroid levels.
Trump lent his name but did not exert management control over the tower's construction and was under no direct legal obligation to conduct due diligence on other people involved.
This data indicate that the clustered soluble EphB4 treatment inhibits the ephrinB2-expressing CNC response to chemoattractant from the placode but does not exert an effect on the placode explant (Fig. 6h, Supplementary movie 5).
It was shown that NPs with a size of 50 nm easily diffused through the cell membrane but did not exert their cytotoxic action (it was supported by high cell survival rates and normal ultrastructure at a concentration up to 500 μg/mL).
USC controlled the game, but did not exert the level of domination expected.
More suggestions(18)
but do not secure
but do not conduct
but do not provide
but do not exercise
but do not enforce
but do not engage
but do not implement
but do not serve
but does not exert
but do not fuse
but do not call
but do not threaten
but do not feed
but do not bet
but do not cross
but do not tiptoe
but do not expect
but do not worry
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com