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Quantification of ZFP57 using isoform-specific primers or primers spanning exons 3/4 to capture both isoforms revealed low but detectable expression in PBMCs, ES cells and several adult tissues, notably the thymus and kidney (Supplementary Figure S1).
When examined in the validation set, miR-1 exhibited similar expression pattern, showing nonexpression in both the preterm infants and children but detectable expression in the adults (Fig. 2B).
Examination of FoxP3 expression by Western blot showed low but detectable expression in P8(1) clone when resting and after nonspecific anti-CD3/CD28 stimulation, but it was amplified after activation with the cognate antigen, Rh protein.
This large group of understudied vaccine targets represents (as the name implies) self proteins with limited or low but detectable expression in many normal healthy cells and tissues and overexpression in multiple to most cancers.
After 2 weeks under LDs, HvFT1 showed a low but detectable expression in Bowman and Bowman eam5), which was significantly lower than HvFT1 expression in Bowman(Ppd-H1) and Bowman(Ppd-H1 + eam5).
The original LAMTOR1 copy was expressed in all bowhead whale adult tissues for which we have data, with the duplicate having much lower (but detectable) expression in heart and retina.
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After heat shock, we observed strong mCherry expression in the lens and weak, but detectable, expression of mCherry in other embryonic tissues (supplementary material Fig. S2, second right panel).
The reduced, but detectable, expression of Kirrel-2 in the transgenics thus indicated that dnRAR did not abolish CNG channel function completely.
There is robust EGFP expression observed in HFFs and significant levels in differentiated EBs and neural-lineage cells, but no detectable expression in hESC or the hiPSC.
There were six miRNAs that were undetectable (zero reads) in normal HOSE cultures (Figure 4C), but had detectable expression in OEC, OSC and/or OCC in the 454 sequencing data, and they likewise showed increased expression in ovarian histologic subtype samples versus normal primary HOSE when examined by TaqMan qRT-PCR (Figure 4C).
For example, eight ncRNAs of group 4 showed conserved sequences but no detectable expression in human tissues (Table 1 and Additional File 5).
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