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Due to the rapid yet transient burst of expression of nec-mRNA, off-target effects will be minimized when compared to long-term expression by viral or plasmid vectors.
It also offers an explanation for how a burst of expression of many Mef2 target genes at a specific phase (stage 13) of the differentiation program is coordinated.
In D. melanogaster, the burst of expression of let-7-5p in prepupae is important to ensure appropriate remodelling of the abdominal neuromusculature during metamorphosis [ 38].
The burst of expression of third instar cuticle proteins could be associated with larvae that are preparing for the stresses of parturition, wandering and pupation.
In addition to detecting a previously unknown early burst of expression of several carbohydrate-associated genes, our data highlight the key role of β-amylases, which undergo an early enhanced expression.
Davis et al. [ 29] showed that the strongest negative relationship between expression profile and evolutionary rate occurs at a late stage in embryonic development, soon after the main burst of expression of segment polarity and Hox genes.
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The burst sequence, approximately 50 bp long, is located between TAAG and the translation initiation site and is required for the burst of expression from the polh promoter.
One of the most peculiar patterns was that of miR-252-5p miR-252-5p miR-252-5p of expression towhich the end of N5, approximately one or two dayshowedr the peak of 20E, and then the expression steadily decreased during most of the N6, including the days of the peak of 20E of this stage.
It is expressed as stochastic bursts of expression, time averaging buffering the burst, propagation, and eventual amplification of fluctuations.
Time has passed, punctuated by regular bursts of expression.
The patterns of the remaining miRNAs examined show two bursts of expression.
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