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The controlled solvent burst method has shown the advantage over the crystal leaching method in the direct creation of microspheres with large closed pores, by atomizing the polymer solution in controlled temperature conditions.
This was the expected bias due to microbalance hysteresis in the burst method when using droplet numbers near the LOQ.
Alternatively, determining the average mass per burst, as outlined in the pulsed burst method, accounts directly for mass differences in the droplets dispensed.
The uncertainties in the burst method data increased significantly when attempting to measure less than a few thousand drops, and mean values also decreased.
As expected, average droplet masses determined by the burst method exhibited no height influence, although scatter appeared greater at the extremes.
The LOQ of the burst method was restricted by the limitations of the microbalance rather than by the variance in the evaporation measurements.
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Note that the continuous droplet and pulsed burst methods depend on droplet or burst ejection frequency and not on the total number of droplets or bursts deposited.
As before, two-sided t tests allow us to conclude that, at 95% confidence, the droplet mass values obtained by continuous droplet and burst methods were equivalent statistically.
Results from dimensional analysis and from the continuous droplet and burst methods, all obtained by measurements replicated across different days with operational variables nominally identical, were compared to test for systematic differences in the determination of droplet mass (Table 2).
Here, we developed a new software, Spiso-3D, to extract spines as well as dendrites in neuronal image based on their geometrical features (Lindeberg 1990; Schmitt et al. 2004), a completely different approach to the ray-bursting method (Wearne et al. 2005; Rodriguez et al. 2008).
The burst coloring method achieved better quality of colors than the nonburst method (Fig. 11b).
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