Firstly, four cDNA libraries were built from total RNA extracted from apical buds harvested during endo- or ecodormancy, from two populations with different bud burst dates (early vs. late flushing populations).
Clonal (or family-wise) mean bud-burst occurred between day 117 and day 131 in year 3 (Fig. 1) but no significant differences between geographic origins were found in the mean bud-burst date (Table 3).
Date of bud burst and date of apex abscission were expressed as day of the year (DOY), i.e. number of days since January 1. Plants were cut back in January 2009.
On March 19 , 2008 NASA's Swift spacecraft alerted astronomers to the brightest gamma-ray burst observed to date.
Each conversation was a short little burst, like speed dating.
The year is starting with a burst of speed-dating among the leaders of the euro zone's three biggest economies.
Still, out of the blue, and with just 24 hours before the formal reply date, Gerta burst into Room 118A announcing she had changed her mind.
Our study documents a careful analysis of a group of pseudogenes in multiple organisms, contrasting against recent studies devoted to draft pseudogene annotation of individual genomes and attempting to date the burst in retrotransposition [ 28, 42].
After all, Louie loved nothing more than to disrupt its observational comic plots with bursts of surrealism – a date escaping via helicopter, a homeless man getting decapitated.
These bursts vary both in date and copy number among families, revealing that each family has a particular amplification history.
