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The Norrin binding site on Fz4CRD (∼800 Å buried area) overlaps with site 2 on Fz8CRD (∼400 Å buried area; Figure 8A), in agreement with previous mutational mapping studies (Smallwood et al., 2007).
Table 1 lists the surface buried area for each of these four FN4 domains to contact the FN5-FN6 entity shown in Fig. 1E.
Table 1 The surface buried area of the four DCC FN4 domains on FN56 FN4-A 1,106 Å2 FN4-B 144 Å2 FN4-C 748 Å2 FN4-D 195 Å2 Note: Out of the 4 potential FN4 domains that pack around FN56, the FN4-A has the largest surface buried area indicating that it makes the largest contact with FN56.
The data for buried area shown in the figures was averaged over a 200-ns window.
Collimated spectrometer measurements of buried area sources demonstrated the ability of the method to yield accurate depth information.
The interface is formed between residues 47 and 71 of the neighboring chains A and D, with the buried area occupying 549 Å2.
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President Clinton talked of it at a news conference at which he pledged federal aid for the buried areas.
Their buried areas are 150.5 Å and 113.3 Å respectively.
Phosphorylation of S174 enlarges their buried areas by 12.1 Å and 8.5 Å respectively, which results in an enlarged Atg31/Atg17 interface (Fig. 5E).
Figure 2C shows a significant reduction in the surface area buried within the dimer interface, consistent with the buried areas in the crystal structures of the ligand-free dEGFR dimers.
The particularly large ΔASA peaks for K382 in complexes with CBP and sirtiun (indicated by the hatched bar) are due to extra buried areas arising from the acetylation of this residue.
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