However, the gadB2-expressing strain under the PdnaK −3) promoter that prolongs to upstream 240 bp grew and consumed glucose much slowly than PdnaK-B2, perhaps due to the reason that tedious fragment might increase the growth burden of bacteria.
The design of vaccine candidates for OM that currently focus on preventing colonization are predicated on the assumption that by reducing the burden of bacteria present in the pediatric nasopharynx, one could reduce or eliminate the likelihood of retrograde ascension of the Eustachian tube by bacteria from the nasopharynx to the middle ear.
However, stable patients receiving prolonged mechanical ventilation without clinical pneumonia have a high alveolar burden of bacteria [ 42].
This could have underestimated the burden of bacteria in our sample and the co-detection of viruses and bacteria remains an interesting issue for further studies.
Another theory is that such a significant intestinal burden of bacteria and high-fibre feed could result in increased oxygen consumption and local bowel ischaemia.
Although colonization of dental plaques with pathogenic bacteria may be a precursor for the disease, chlorhexidine based decontamination of oral microbial flora alone might not be sufficient to reduce the burden of bacteria responsible for NP.
Burdens of the bacteria after molting or after oviposition were estimated by quantitative polymerase chain reaction with primers and probes specific for each bacterial species or, as an internal standard, the host tick.
The ratios of the fusA gene of the Coxiella sp. to the MIF gene of the A. americanum in a given tick were used to estimate the burdens of the bacterium in the ticks at different stages of their life cycle.
These findings were further evidence that the Rickettsia sp. was not as prevalent as the Coxiella sp. in the ticks at any stage, and among ticks with the Rickettsia sp. the burdens of this bacterium were considerably lower than the Coxiella sp. In the first experiment nymphs were injected with PBS alone or PBS with tetracycline.
Tube feeding might increase gastric burden of pathogenic bacteria and predispose patients to ventilator-associated pneumonia.
The growing danger of life-threatening infections and the rising economic burden of resistant bacteria have created a demand for new antibacterial therapeutics.
