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The total pain burden was quantified using an area-under-the-curve (AUC) method [ 14, 43].
To address this question, mice were immunized with AdM42 or with the previously described vaccines expressing MSP-142 fused to mouse complement C4b-binding protein (C4bp) (Draper et al., 2008) and were challenged with 5000 sporozoites, and the liver-stage parasite burden was quantified using a real-time RT-PCR assay.
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The burden of comorbidities was quantified using the Charlson comorbidity index [ 16].
Forty hours after sporozoite inoculation, the livers of all mice were harvested, total RNA was isolated using Tri-reagent (Molecular Research Center, Cincinnati, OH) and the parasite burden in the liver was quantified using reverse transcription (RT) followed by real-time PCR as outlined in Bruna-Romero et al. (2001).
The atherosclerotic burden of the abdominal aorta was quantified using a calcium scoring method.
Information about patients' age, sex, ASA grade, burden of general morbidities (which was quantified using the Charlson co-morbidity score [ 18]), BMI, indication for and the nature of the procedure undertaken, and Mallampati [ 19] and Cormack-Lehane direct laryngoscopy grades [ 20] were recorded.
Within the context of a national population health survey, the burden of illness for persons with MS was quantified using a generic health measure, HUI3 in the community dwelling population.
Fluorescence was quantified using ImageJ.
Cell size was quantified using SigmaScan Pro.
OPA1 fragmentation was quantified using AIDA software.
DNA was quantified using ultra-violet quantification.
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