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Disease burden was determined using the Newcastle Mitochondrial Disease Adult Scale NMDASS) and urinary mutation load.
The significance of differences in fungal burden was determined using one-way ANOVA and Dunnett's multiple comparison tests.
The significance of differences in fungal burden was determined using one-way analysis of variance (ANOVA) and Dunnett's multiple comparison test.
Plaque burden was determined using volumetric analysis: mm of index lesions (0.5±0.02; n=67) for all patients on a slice-by-slice basis.
In this study, the importance of musculoskeletal disease in terms of economic burden was determined using a number of data sources such as NHIC statistics, the Korea Health Panel study, and cause of death reports generated by the Korea National Statistical Office NSOO).
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Practicability and burdening were determined using 5-point Likert scand, and open questions, vital signs, and time investment.
The hepatic and splenic parasite burdens were determined using a quantitative limiting dilution assay.
The percentage tumour burden within the liver was determined using the baseline CT/MRI scan, utilising validated tumour volumetry software (MeVis Distant Services, Bremen, Germany).
Lung burden of the remaining time points was determined using the whole (not lavaged) lung.
For both survival assays, Kaplan-Meier survival curves were plotted using GraphPad Prism 6.0 (GraphPad Software, La Jolla CA) and significance was determined using a log-rank test, whereas organ burden significance was determined using the Student's t-test.
Difference in pathological scoring parameters between groups was determined using the Fisher Exact test, and differences between bacterial burden scores and differences in skin-test responses were compared using the non-parametric Mann-Whitney test with Bonferroni post-test analysis.
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