The filling material was removed coronally to the extent of 2 mm deep by using a cylindrical diamond bur number 50 (KG Sorensen, Cotia, SP, Brazil).
A small hole was carefully made in the center of the incisor tooth in order to expose the dental pulp using a dental bur (round bur number 3, Meisinger, Neuss, Germany).
Root canals were prepared using the step-back technique with Ni-Ti K-files (K Files NITI FLEX, Dentsply, Germany) up to number 50 and Gates Glidden burs (numbers 2, 3, and 4) (JS Dental, Ridgefield, USA) which allowed entrance of the laser fiber to the apical area.
DIS _{ exp, bur } Number of the predicted exposed/buried disordered residues divided by the number of exposed/buried residues (2 features).
The reduced ovule number in Bur is also consistent with results demonstrating that overexpression of the pea GA catabolism gene GA2ox2 in arabidopsis resulted in reduced ovule number (Singh et al., 2010).
In contrast, both seed size and number for the Bur ecotype were significantly different (P < 0·01) in the two environments (Table 1).
The reduction in ovule number seen in Bur grown in the higher temperature Cvi environment is consistent with the observation that heat stress reduces ovule number and increases abortion in arabidopsis (Whittle et al., 2009).
These results suggest that, while the number of enteroendocrine cells expressing burs is rather constant, the levels of burs within these cells are regulated.
{ EXP, BUR }_ RES _ seg {1,5,10,15,20} Number of the predicted exposed/buried residues in the exposed/buried segments which are at least 1, 5, 10, 15 and 20 residues long divided by the sequence length.
The samples canals were enlarged by using number 2 through number 5 Gates-Glidden burs (Mani, Tochigi, Japan) to form 1.3 mm diameter standardized spaces.
The canal spaces of the root slices were instrumented by number 2 through number 5 Gates-Glidden burs (Mani, Utsunomiya, Japan) to form 1.3 mm diameter standardized cavities.
