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Mean actin filament bundle width was significantly increased by 72 h hypoxic incubation (Fig. 2e).
Hypoxia significantly increased mean actin filament bundle width (72 h) and actin filament length (72 96 h).
Using a linear-feature detection algorithm [ 42] we quantitated various cytoskeletal parameters, including actin filament bundle width and length (Fig. 2e g).
Actin filament bundle width and length were quantitated using a linear-feature detection algorithm developed in collaboration with the CSIRO and previously described [ 42].
A dynamic actin cytoskeleton is necessary for enhanced migration and invasion [ 28] and it is likely that our observed hypoxia-induced strengthening of actin cytoskeletal organization increased actin filament bundle width and length led to reduced actin dynamics.
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The resulting six-helix bundle has a width of 5.5 nm, a height of 14 nm, and an inner channel diameter of approximately 2 nm.
The product is composed of abundant belt-like bundles with a width of 30 100 nm and a length of up to 1 μm.
The resulting six-helix bundle has a nominal width of 6 nm and a height of 14 nm.
Optimal ordering and sizing of wires in a constrained-width interconnect bundle are studied in this paper.
During real-time confocal microscopy, we set the image resolution to 512 × 1024 pixels to cover an area of 5 axonal bundles (100 μm) in width and 200 μm in length (Fig. 1B).
In contrast to blebbistatin-treated cells, cells treated with ML-7 retained prominent actin filament bundles of length and width similar to those in untreated cells (Fig. 2, C and F).
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