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The left renal vascular bundle was ligated with surgical suture and the kidney removed.
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The distal end of the artery was ligated with suture.
The product of PCR was ligated with pPIC9K (SnaBI/EcoRI).
The common bile duct of guinea pigs was ligated with or without MLT pretreatment.
The gene fragment was ligated with the pGK::nucMCS plasmid after digestion with XhoI and SalI.
b Surgical view, showing that IMA was ligated with vascular clip.
The PCR fragment was ligated with EcoR I-Kpn I digested pHA12 (Arai et al. 1991) to construct pHA12-dhaB.
The proximal facial artery was ligated with vascular clips, the lingual nerve was separated from the submandibular ganglion with monopolar cautery, and Wharton's duct was ligated with a vascular clip.
The needle wound was ligated with cotton thread.
The right MCA was ligated with a l0-0 nylon suture.
Low molecular weight RNA was ligated with 5' and 3' adapters separately and gel purification of the adapter ligated cDNA was done.
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CEO of Professional Science Editing for Scientists @ prosciediting.com