At the beginning of the metaphyseal flare (Fig. 7), the posterior tibial neurovascular bundle moves from a midline location to posteromedial.
The position of the pipette and bundle were continuously monitored before, during and after each recording using video microscopy to ensure that the stimulus pipette and the hair bundle moved in unison.
Evidently, the whole bundle moves as a rigid body from its position in protomer A to that in protomer B, while the central dimer contact domain remains static.
The inward-open model of TtNapA implied that the 6-helix bundle moves up and down by 10 Å and rotates by 21° about an axis roughly perpendicular to that in MjNhaP1 and ASBT.
Prior to the publication of the alafosfalin studies, a comparison between the occluded PepTSo structure and inward open PepTSt revealed that helices in the C-terminal bundle moved away from their respective positions in the occluded state to open up the central binding site (Fig. 8A) [49].
Judge Jackson ruled that Microsoft's bundling move and its behavior did violate antitrust laws, and Microsoft is appealing his ruling.
After 31.1 ± 1.36 min (n = 20), directed movement of filament bundles towards the center of the ring (the moving equator) ceased, and only filament bundles moving nearly parallel to the direction of the shifting ring could be observed (yellow arrowheads, Figure 2E).
Tom Miller, the attorney general for Iowa, one of the states that joined the Justice Department in suing Microsoft, said in an interview that the decision "clearly calls into question" the software bundling moves Microsoft plans to make in Windows XP, which is to be introduced in October.
The direction of the displacement, however, was variable: 21 hair bundles moved in the positive direction, toward the kinocilium, whereas seven bundles moved in the opposite direction (Fig. 4B and C).
In the first 15 min of blebbistatin treatment, the filament bundles moved at a velocity of 7.62 ± 0.78 nm/s (n = 18), which was significantly slower than the velocity in control cells (DMSO-treated cells) (29.92 nm/s, n = 10; p < 0.001), where the filaments moved towards a stationary equator.
