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The control experiments were also carried out by using bull serum albumin (BSA) and lysozyme in the absence of thrombin.
Bull serum albumin (BSA), fluorescein diacetate (FDA), and propidium iodide (PI) were purchased from Sigma (New York, NY, USA).
In this study, a simple, mild, and green approach was developed to synthesize cerium-doped carbonaceous nanoparticles (Ce-doped CNPs) using bio-mineralization of bull serum albumin (BSA) as precursor.
Herein, we developed novel SERS probes, based on BSA (Bull Serum Albumin) coated gold silver core shell nanorods modified with Raman reporter 5,5-dithiobis 2-nitrobenzoic acid (DTNB) (Au@AgNRs@BSA@Anti-MICA), for in vitro cancer cell detection.
Resolved proteins were transferred onto a polyvinylidenedifluoride (PVDF) membrane (Millipore, Billerica, MA, USA), and then blocked for 1 hour at room temperature using Tris Buffered Saline Tween (TBST) with 5% Bull Serum Albumin (BSA).
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Bulls supplemented with PE had greater (P<0.05) serum insulin concentration than MON or CTR bulls.
Leptin serum concentration increased (P<0.01) from 35 to 71 days of study; however, in MON bulls this increase was not as pronounced as in PE and CTR bulls.
Bull sperm.
There was a strong correlation and association between serum testosterone and NO levels besides sexual behavior of buffalo bulls.
Additionally, serum concentrations of TNF-α were greater in heifers than bulls from 1.5 to 2 h after the LPS challenge.
We studied the effect of sexual excitation on serum testosterone and nitric oxide (NO) levels in water buffalo bulls with different categories of sexual behavior and their correlation with each other.
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