Specifically, the correlation of thermo-physical events during vitrification of carotid artery segments with function recovery is reported using marginal thermal conditions for achieving vitrification in bulky samples.
A novel strategy combining (2,3,4,5,6 -pentafluorostyrene (PFS) grafting and subsequent thiol/para-fluorine "click" type post-grafting modification is presented to impart specific surface properties to bulky PVDF samples.
Tissues were immediately stored in RNAlater, with the exception of whole body tissue, which was immediately frozen at −80° in case RNAlater solution did not readily permeate the more bulky body samples.
Although a routine, rapid, and simple determination of Hcy levels is highly desired, the existing methods are practically limited because of complicated sample preparation and bulky instrumentation.
The approach required neither sample preparation nor bulky instrument, suggesting the point-of-care testing of Hcy levels, and is potentially useful for routine, cost-effective, and precautionary diagnosis of various cardiovascular diseases.
Methods for the measurement of lactate in the blood possess the same disadvantages as measuring cortisol; taking blood samples, expensive and bulky laboratory equipment, and lengthy processing time make lactate monitoring difficult [8].
However, such standard methods require expensive and bulky laboratory equipments, analytical expertise and sample transportation, and generate hazardous wastes [ 10, 11].
We analyzed 4 thick PM's (>4 mm) and 16 MM samples composed of 12 bulky, macroscopic (replaced) lymph node metastases and 4 subcutaneous metastases.
We analyzed 40 MM samples, composed of 22 bulky, macroscopic (replaced) lymph node metastases, 16 subcutaneous and 2 solid organ metastases (adrenal and brain), and compared them with 42 primary cutaneous cancers [16 PCM, 11 SCC, 15 BCC].
However, these approaches require complex sample preparation procedures, expensive and bulky instruments, and professionally trained personnel running the tests.
