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The normal Raman spectrum that was obtained from the BPE methanol bulk solution with a concentration of 10− 2M, and the SERS spectrum of the glancing-deposited Ag NRA were measured.
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In the absence of additional electrolytes, NPEs exhibited substantially higher activity in micelles than bulk solution; with growth of EO groups, shrinkage on the scale of synergistic interaction was evidenced.
This peculiarity of the system leads to the anomalous dependence of the maximal current density of the bromate reaction on the electrode size: if the electrode radius is under its critical value the current density is very small since it is determined by the discharge of bromine species from the bulk solution, with no significant transformation of bromate.
These results collectively demonstrate the high level of efficiency and specificity of the Genomic Assay® when practised in bulk solution with 25-mer ssDNA probes and YOYO-1, to directly detect mismatches between probes and non-denatured, non-amplified human genomic dsDNA targets, over a broad range of genomic target concentrations.
Measured cleavage rates were the same as those in bulk solution, with DNA topology having no effect.
The effect on the deposition of dilution of the bulk solution in contact with the surface was also investigated by gradual replacement of the bulk solution with 1 mM aqueous NaCl.
A subsequent dilution with 1 mM NaCl removed additional material from the surface, but the final adsorbed amount was higher than on directly diluting the bulk solution with 1 mM NaCl.
2), Screening of the enzyme from the surface and nonspecific interactions with biomaterial in bulk solution requires a grafted layer composed of short hydrophilic polymers and long triblock copolymers.
As an external electric field is applied, the positively charged ions move along the electric field and drag the bulk solution moving with them, yielding an electroosmotic (EO) flow (see Figure 1a).
This solid phase is not in equilibrium with the bulk solution and as a result, chemical dissolution will take place.
Firstly, binding from the bulk solution is probably rapid with a rate equal to a sizable fraction of a diffusion-limited reaction.
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