Bulk sequencing of such a population will report a normal genome, since the rare mutants would be swamped by the germline sequence of unaffected cells as well as the normal allele in a mutant cell.
The resistance tests analysed in this study used bulk sequencing of the pol gene, encoding the protease and reverse transcriptase genes using a variety of in-house and commercial assays.
Therefore, a shift in focus emerged, from complete metagenome sequencing to bulk sequencing of as many possible genes and/or functions.
A salt stress-related gene, TaMYB32, was screened out during the bulk sequencing of full length of cDNAs in wheat (Triticum aestivum L). that were cloned in the authors' laboratory.
Bulk sequencing of one sample showed nucleotide mixtures in the RT at the resistance-associated codons G190G/A, L210L/W and an undecipherable mixture at codon 215 in reverse transcriptase (sample 'A') (Figure 4A).
The fraction of ambiguous nucleotide calls in bulk sequencing of human immunodeficiency virus type 1 (HIV-1) carries important information on viral diversity and the age of infection.
The resistance tests analysed in this study used bulk sequencing of the pol gene, encoding at least codons 4-99 of the protease gene and 34-234 of the reverse transcriptase gene, using a variety of inhouse and commercial testing systems.
The extent of editing site conversion of the six editing sites associated with CRR4, CRR21, CLB19, and OTP82 was determined in 21 species using bulk sequencing of cDNA (Table 1).
Genomic approaches were first accessible to population geneticists via targeted amplification of genome-wide markers (Vos et al. 1995), but the declining cost and increasing length of next-generation sequencing reads are now making bulk sequencing of the genome practical for allele discovery in nonmodel and outbred study subjects (Li et al. 2008; Davey et al. 2011; Nielsen et al. 2011).
To assess the impact of HLA-A*03-mediated T cell selection pressure, we determined the bulk sequence of the non-structural region of the viral genome in the serum of HLA-A*03-positive and -negative patients derived from the cohort (table 1 and supplementary table 8).
