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If the large BFR is caused by different allele frequencies at the DNA level in the two bulks but not by allele-specific expression, the ratio of the two alleles in the combined bulk (resistant bulk plus susceptible bulk) should be relatively small and predictable with the family structure.
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Overuse, under-use, poor diagnostic techniques and inappropriate choice of antimicrobial account for the bulk of resistant strains emerging world wide.
Two parents (RP and SP) along with two phenotypically contrasting bulks, one resistant and one susceptible to PM and each comprising 50 F2 plants were generated.
Two phenotypically contrasting bulks, one resistant (BR) and one susceptible (BS) to P. halstedii and each comprising 16 F2 plants were generated.
A sunflower transcriptome reference assembly (BRBS) was developed by combining reads from the resistant bulk (BR) and the susceptible bulk (BS).
Sequence libraries of one resistant bulk (BR) and one susceptible bulk (BS) each containing pooled cDNA of 16 individual F2 plants were generated to perform the 454 sequencing.
In this way, the bulk basalt becomes resistant from further alteration.
Infected cells were selected with 0.5 µg/mL neomycin for 12 days and the bulk of the resistant cells was used in subsequent experiments.
Due to the sensitivity of microarrays, plant-to-plant variation of gene expression could be reduced by bulk harvesting of resistant and susceptible hybrids.
We have successfully adapted standard BSA procedure to our particular conditions: 1) since we could not easily discriminate susceptible insects in the BC offsprings we have only used one of the bulk extremes (the resistant insects).
Transfected cells were grown as bulk selected hygromycin-resistant cultures at 37°C in OPTI-MEM with 5% calf serum supplement, iron-enriched (GIBCO-BRL) and 500 μg/ml Hygromycin B (Calbiochem, La Jolla, CA).
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