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Until recently, molecular mediators of memory formation were studied in bulk populations.
After the discovery and early characterization of MSCs, scientists desired a method to prospectively isolate progenitor cells from bulk populations based upon positive or negative selection of CD markers expressed by the cells.
Previous studies of C. albicans and immune cell interactions in bulk populations have identified key pathways by characterization of either the fungal or host transcriptional response during these interactions3,6,7.
Prior microarray analysis of bulk populations of C. albicans exposed to macrophages reported similar changes3; by sorting infection fates, our work suggests that this response is specific to engulfed C. albicans, allowing the pathogen to utilize the limited spectrum of nutrients available in the phagosome3.
The results of these assays therefore provide best estimates of transduction efficiency and gene expression in bulk populations based on the assumption that gene transfer and expression are uniformly distributed among transduced cells.
Brenner also chose it as it is easy to grow in bulk populations, and convenient for genetic analysis.
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For qRT-PCR of bulk population samples, 10 ng of total RNA was used for each biological sample.
The chief advantage of the bulk population method is that it allows the breeder to handle very large numbers of individuals inexpensively.
(A) The use of such stimulation has historically been limited to perturbative paradigms, wherein pulse-type inputs are used to create bulk population responses without fine temporal structure.
However, a bulk population of the cells (at least 5 × 105 1 × 106/ml) should be prepared as sorting material, which is greatly limited in accommodating low-abundance cell subpopulations.
Here, surprisingly, one population of cloned mMSCs showed an even better reconstituting potential than the bulk population.
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