Sentence examples for bulk passage from inspiring English sources

Suemori, H. et al. Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.

The patient did not initially mention it to her treating physician, noting only bulk passage of tissue (as opposed to gradual sloughing) in response to a physician query after the fibroid had been noted to have essentially disappeared on the 3-month MRI study.

rES cell lines were stably maintained by the bulk passage method [12] using both collagenase treatment and disaggregation of colonies by pipetting using Pipetman P-1000.

These predictions were experimentally verified in arrays of microchannel constrictions with precisely engineered dimensions and in bulk passage through filters with well-defined pore size.

For bulk passaging, neurosphere cells were mechanically dissociated and replated at 10 cells/µl in a 24-well plate.

However, it has been shown that other culture conditions, including feederfree conditions and enzymatic splitting that are commonly used for the maintenance of undifferentiated hESCs, are associated with accumulation of genetic and epigenetic alterations with bulk passaging and extended time in culture (Baker et al., 2007; Imreh et al., 2006; Maitra et al., 2005).

Chromosome preparation and spectral karyotyping of eGFP-transduced bulk-mMSCs, passage 13 and clone IXH8, passage 20 were performed as described [47].

Should a strong southwesterly wind prevail and allow the fleet to hoist spinnakers for the bulk of the passage, there are several boats with a shot at beating the course record of 33 hours 29 minutes 57 seconds.

Since Immortalization is expected to perpetuate the ability of stem cells to self-renew [29], we subjected T-IhNSCs neurospheres to long-term, sequential passaging as bulk cultures, in order to determine their extensive self-renewal capacity and related steady growth properties.

For experiments using neurosphere-derived NPCs, bulk cell cultures were passaged by pelleting the sample and mechanically dissociating the pellet using a small borehole pipette and growth factor-supplemented SFM and plated as described.

Gsk3 deletion or inhibition is sufficient to maintain ESC self-renewal in bulk culture for several passages, albeit with ongoing differentiation (Doble et al., 2007; Wray et al., 2010; Ying et al., 2008).