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Based on charting frequencies, a discontinuity is seen in expression following these top four transcripts, with notably reduced levels among the remaining bulk of transcripts.
However, in the bulk of transcripts obtained from RiboZero data, it remains unclear how to differentiate between transcripts that are polyadenylated, not polyadenylated and/or bimorphic [ 7].
In the bulk of transcripts identified in the RiboZero procedure, it remains difficult to differentiate a priori between polyadenylated and not polyadenylated, or bimorphic transcripts.
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Yet for most organisms, the bulk of transcript evidence comes in the form of ESTs as they are less expensive to produce in a high-throughput manner than full-length mRNA sequences.
These transcripts are distinct from the bulk of transcription that occurs in the active LCR and δβ sub-domains that occurs predominantly in G1 phase.
In addition, identification of a gene responsible for a specialized function during a certain biological stage can be difficult to determine because the gene might be expressed at low levels, whereas the bulk of mRNA transcripts within a cell are abundant [ 7].
Among these is the target region with highest NLQ value (#1203), which was located upstream of the ORF alr2921, with a GTGN8TAC sequence separated 35 nucleotides from a bulk of 5′-transcript ends activated in combined-N free medium [ 13], which could represent a putative TSP, preceded by a -10 box.
Table 5 shows that 69% of the TARs have EST matches, and 43% of the TARs have matches in the human cDNA library, which further validated that the bulk of the novel transcripts identified from tiling arrays are real transcripts instead of experimental artefacts.
The bulk of commonly regulated transcripts (55%) followed the pattern: down-regulated under low temperature and low N, but up-regulated under low P conditions.
Similar to what has been recorded in normal Leydig cells, we first found the bulk of tumour CYP19 transcripts to be PII derived, excluding promoter shift as a cause of enhanced transcription.
This notion has been suggested by many, including the researchers who conducted the tiling array experiments, as the most likely explanation for the bulk of the novel transcripts [ 9, 11, 16].
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