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simple powder mixing, using a mortar and a pestle; kneading with a 50% (w/w) water : ethanol solution until the bulk of solvent evaporated and a paste-type product was formed; the mixture was then dried at room temperature for 24 hours and put in the oven, at 105°C, for several hours until reaching constant weight.
Different methods were applied for the preparation of the inclusion complexes: simple powder mixing, using a mortar and a pestle; kneading with a 50% (w/w) water : ethanol solution until the bulk of solvent evaporated and a paste-type product was formed; the mixture was then dried at room temperature for 24 hours and put in the oven, at 105°C, for several hours until reaching constant weight.
The CD and the active compounds were kneaded with 50% (v/v) water : ethanol solution until the bulk of solvent evaporated and a paste-type product was formed; the mixture was then dried at room temperature for 24 hours and put in the oven, at 105°C, for several hours until reaching constant weight.
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Of the two solvent descriptors, the ΔvapUO indicates the energy involved in the solute cavity formation in the bulk of the solvent in the dissolution process and can also be associated to the nonspecific solute/solvent interactions.
It takes some time before the ion-concentration within the bulk of the solvent is affected by the dissociation or association of condensed ions.
Therein the organic solvents utilized are dispersed from the bulk of the solution, leading to the extraction of the solvents and the precipitation of the particles.
In this context, the influence of proton activity in the mobile phase and the effects of varying concentration and type of the counterion as well as type of co-ion and of bulk solvent components were thoroughly investigated.
Essentially, assuming Brown relaxation the rotational energy of the whole particle is transformed into rotational, vibrational and translational energy [19] of the surrounding nanoscopic layer of bulk solvent and aspirin molecules, thereby increasing the hydrolysis rate.
Our desolvation kinetics data obtained from running free energy perturbation also corroborates the assumption in theory that greater the loss of bulk solvent at the interaction interface of ZFP-DNA complexation stronger the binding affinity and stability of the complex.
The PES scans were performed using various combinations of DFT/coupled-cluster methods and basis sets, and the effect of bulk solvent was examined by using the integral equation formalism of the polarizable continuum model (IEF PCM).
The effect of bulk solvent was considered through the polarisable continuum model, by using a relative dielectric constant of εr=4.9 for chloroform.
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