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We extracted DNA from a bulk collection of leaf material from Queensland, Australia, which originated from American species of sect.
You're after the bulk of the leaf to improve the soil consistency, but you could leave a few to flower, as they are great for insect life and pretty, too.
DNA was extracted from a bulk of young leaf tissue of 20 30 plants per accession as previous described [ 34].
The DNA extraction was performed on a bulk of one leaf from at least 10 three-week-old plants per line following the methods described by Guidet et al. (1991) with minor modifications.
The bulk of the other leaf proteins were accumulated in supernatant (S) and in the microsomal F1 fractions.
The spatial patterns predicted by the Two-pool and Péclet models of bulk leaf water were similar to those shown for the sites of evaporation model (see Figure 2 for latitudinal trends in δ18O for all models).
We evaluated two alternative models of "bulk" leaf water that have been developed to explain this discrepancy.
The wild-type and mutant bulks for both populations were composed of leaf tissue from 20 green and 20 yellow plants, respectively.
Although the stable carbon isotopic composition of each biochemical fraction in the fresh leaves are different compared to that of the bulk leaf, the tall and dwarf leaves can be distinguished on this basis.
However, the δ13C and δ15N of the bulk leaf, the biochemical fractions, and seven amino acids can be used to distinguish dwarf and tall trees at Twin Cays, BZ.
Equal amounts of leaf tissue from each population were bulked before extracting RNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com