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Although some biochemical studies of Syt1 have been conducted on brain extract or mammalian cell line immunoprecipitated protein, the bulk of biochemical characterization, which requires larger amounts of Syt1, has been conducted on the Escherichia coli-expressed C2AB fragment of Syt1 consisting of only C2A and C2B domains.
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The high energy and capital investment often fatally weaken the economics and sustainability of bulk biochemical production.
All of the findings above raise the need for caution in the interpretation of biochemical assays conducted using bulk purifications of TERT complexes.
Because the bulk of our current genetic and biochemical knowledge of autophagy comes from seminal early genetic screens in yeast, it is not clear to what degree newer functions of autophagy rely on metazoan-specific signaling pathways.
Although the bulk of the claims based on biochemical analysis are well supported by genetic studies, direct evidence of interactions and covalent modification of endogenous proteins would strengthen the paper.
The results of the bulk biochemical analysis may explain the contrasting findings.
It is important to note, however, that our measurements of bulk properties and biochemical constituent content may not detect compositional changes to the matrix.
Over several decades, a variety of biochemical assays have been applied to bulk bacterial cultures.
The developed integrated enzyme logic gates produce local pH changes at the EIS sensor surface as a result of biochemical reactions activated by different combinations of chemical input signals, while the pH value of the bulk solution remains unchanged.
However, the δ13C and δ15N of the bulk leaf, the biochemical fractions, and seven amino acids can be used to distinguish dwarf and tall trees at Twin Cays, BZ.
It is well established from bulk biochemical assay of whole brain homogenates that lactate levels increase rapidly during the postmortem time interval, reaching a maximum within 2 3 min of ischemia.
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