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Recently it was validated as a diagnostic target for the detection of MAP in a large number of bulk milk samples obtained from MAP infected herds [15].
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Breast milk samples obtained at 3 months postpartum were analyzed for PBDEs.
The aim of the current study was to investigate the occurrence of mycobacteria in bovine raw-milk samples, obtained from both individual and collective bulk tanks and informal trade in the southeast region of Sao Paulo, Brazil, using microbiological cultures and polymerase chain reaction (PCR -based testing.
Herds were assigned a salmonella status on the basis of antibody analysis of bulk milk samples.
Twenty-four bulk milk samples were positive for mycoplasma over the course of the monthly portion of the program.
In this study, the isolation of 52 mycobactin-independent fast growing mycobacteria from 631 bulk milk samples (8.2%), is reported.
The study materials were bulk milk samples collected from market areas of the mentioned towns.
The bulk milk samples were centrifuged and stored at −20°C and were retrospectively checked for antibodies against BTV.
Since the milk IELISA is an imperfect test, we used estimates of sensitivity 0.953 and specificity 0.951 for pooled bulk milk samples from the published literature [30].
For 12 of the 50 dairy herds with BTV antibodies in bulk milk samples, a follow-up analysis of milk health production indicators was performed at monthly intervals.
An indirect BTV antibody ELISA designed for use on bulk milk samples (mELISA) [23] was performed by ARSIA according to manufacturer's instructions (ID.VET, Montpellier, France).
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