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At the end of the recording session single cell electroporation of biocytin was performed on the bulk loaded neurons (typically, 1 4 calcium loaded neurons).
Following this recovery period, ex vivo brains were bulk loaded with OGB-1 AM (Invitrogen, Carlsbad, California, USA).
Slices were bulk loaded with Fura 2-AM for visualization of action potential-related activity in neuronal somata.
(A ) TPLSM Fluo-4 bulk loaded image of the imaged field.
Alignment of the bulk loaded neurons onto the barrel using single elecrtoporated cells.
Cultures were bulk loaded with Oregon Green 488 BAPTA-1 (OGB1 -acetoxymethyl ester as fOGB1 -acetoxymethyl
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To counter act this effect, we have pre-bulk loaded all index structures at once after a reboot (taking roughly 80 seconds) and then performed compression.
Bulk loading and specialized shipping facilities are usual in the mining industry, and extraction methods are considered advanced by international standards.
It revolutionised the transport of goods by abolishing the traditional (and back-breaking) system of "break bulk" loading, and thus helped oil the wheels of globalisation.
Mr. Seeba said the ships affected so far were carrying bulk loads, like grain, cement and steel, as well as oil.
Many goods are carried as bulk loads and on pallets, which can be transported in different ways for maximum efficiency.Box-bound or not, the three biggest express operators are broadening the range of services they offer.
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