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As all identified trGTPases were found to carry the G domain and domain II-homologous regions, the sequence region used to build the MAFFT alignment on which the superfamily tree was built was extended to encompass both these domains (an average length of 331 amino acids).
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Since 1825, when the Stockton and Darlington Railway was built (it was extended to Middlesbrough in 1830), Teesside has been the scene of large-scale industrial development and urbanization.
In 1852 the Camellia House was built; this was extended in 1870 to its final size of 385 feet long, 10 feet wide, and 18 feet high.
Downstairs, you can see the old schoolroom built when the house was extended four years after it was built, the stables, the kitchen, and there's a cosy tea room too.
Only after World War I, when the Boardwalk was built and the subway was extended out to Stillwell Avenue, did the area become a working-class destination.
In 1787 the Frankstown Path (a trail connecting the Susquehanna and Ohio river systems) was surveyed through the area; a road was built that shortly after 1800 was extended to Pittsburgh (80 miles [130 km] west).
The lock keepers' cottages were built in 1827, when the canal was extended to Turf.
The Grade II-listed Old School Room was built by Patrick Brontë in 1832 and was extended in 1850 and 1871.
Carrick House, on East Street was built around 1840 by Roderic O'Conner and was extended in the 19th century.
The M5, which was built with dual two-lane carriageways, was extended southwards, from junction 8 in 1969, with dual three-lane carriageways.
Fatigue testing was extended to strands build of 85 rods and terminated in a special metallic end-fitting.
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