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A median-joining haplotype network for the same dataset was also built using the program NETWORK.
The remaining parts of the model were manually built using the program O and refined with the program CNS [17], [30].
All models will be built using the program MlwiN.
83.9% of the model was built using the program ARP/wARP [ 37].
Instead genealogical information of Brueelia and the other taxa was extracted from a haplotype network built using the program TCS 1.2 [ 50].
The model was furthermore built using the program COOT (Emsley & Cowtan 2004) and then improved by several cycles of manual rebuilding and refinement using the program REFMAC5 (Murshudov et al. 1997).
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Relatedness trees were constructed based on chord distances using the neighbor joining algorithm, and a majority rule bootstrap consensus tree was built using the programs NEIGHBOR and CONSENSE in PHYLIP v. 3.65 [64].
Trees were built using the programs 'protml' and 'nucml' for protein and nucleotide sequences respectively [ 26].
Alignments were built using the MUSCLE program [ 38] and manipulated using the JALVIEW program [ 39].
Therefore, three-dimensional structures of antiandrogens were first built using the computer program Gaussview (version 4.1.2; part of the computer program Gaussian 03) (Frisch et al., 2004) and then geometrically optimized in a quantum mechanical force field at the level of restricted Hartree-Fock (RHF) 6-31g* using the program Gaussian 03.
Neighbour joining trees were built using the NEIGHBOR program, and the consensus tree was derived using the CONSENSE program.
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