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The second set of plasmids, built using inserts from the first, was designed to both simplify and speed up the recombineering procedure and generate high fidelity FP fusion gene reporter products.
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The virtual chemical libraries (Additional files 1 and 2) used for molecular similarity analysis were built using parent drugs and metabolites identified in the package inserts and literature references cited in the previous paragraph and from additional published literature on pharmacokinetics of amphetamine-type stimulants [44, 56 62] and synthetic cannabinoids [25, 30, 33, 40, 63 69].
The clones comprised 10 different libraries, built using either HpaII (50,686 reads) or SalI (30,046 reads), with insert sizes ranging from 7 kb to over 100 kb.
Consider using inserts in your shoes.
You can either insert it using the Insert picture command, or by copy-and-pasting it from somewhere else.
DNA isolated from the filter-trapped microbes was used to build small insert libraries for shotgun sequencing (Materials and Methods).
Migration and invasion assays were performed using transwell inserts (Corning) or Matrigel-coated inserts (Corning), respectively.
In addition, we developed the first Spanish database storing drugs as well as their effects automatically built from drug package inserts gathered from online websites.
Ships built from 1939 1940 used a Scotch marine boiler.
Methodology Schwann cells derived from the sciatic nerves of neonatal rat were co-culured with osteoblasts using Transwell inserts.
The work pieces were prepared from CK45 steel using an insert-type ball-end cutter.
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