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To broaden our knowledge of the genomic nature of solanaceous plants further, we dissected the eggplant genome and built a draft genome dataset with 33,873 scaffolds termed SME_r2.5.1 that covers 833.1 Mb, ca. 74% of the eggplant genome.
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The first step was to build a draft reconstruction.
In order to build a draft reference genome and to develop SNP assays, we sequenced the entire genome of C. vestalis on a single lane of paired-end sequences (2x100 bp) on an Illumina HiSeq 2000 (Illumina Inc., U.S.A).
On the basis of the results from the first round of this pilot curation, the group defined major concepts or root classes on which to build a draft ontology, namely exposure stressor, exposure receptor, exposure event and exposure outcome.
The functions in MicrobesFlux can help users build a drafted metabolic model for further reconstruction and flux analysis.
We first built an initial draft model iAbaylyiv1 using information from the genome annotation, metabolic pathways databases, and the literature.
We built an initial draft of a consensus map with JoinMap using a limited set of quality-filtered markers under conditions that were likely to minimize the number of misplaced loci (Table 2).
Since then they have built a reputation for drafting teams including characters that are often neglected by others.
The goal, Luhnow said, was essentially to build a second draft class, after the one he selected in June.
With this scaffold we built an in silico draft by mapping known enzyme-encoding genes, using gene homology information obtained from Génolevures protein families [ 8, 9] and complemented with other in silico methods, and filled network gaps in order to make it functional (i.e. to be able to predict growth from available metabolites in the media).
JoinMap 3.0 was used to build an initial draft of a consensus map based on the 1,546 'bPb' DArT loci collapsed into 959 bins.
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