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Several additional analyses were made to detect the causes of this genetic structure within clades S and P by building a statistical parsimony network for each.
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The program TCS [ 35] was used to build a statistical parsimony network and subsequently GeoDIS 2.0 [ 36] was used for making an NCA.
Future work will involve building a statistical model of hematomas based on existing CT data.
The program was used to construct a statistical parsimony cladogram from parsimonious connections calculated at Pj≥0.95 and a matrix of absolute pairwise differences was calculated.
A statistical parsimony network of haplotypes was built with TCS software v. 1.2139.
A statistical parsimony network was created using the program TCS version 1.21 (Clement et al., 2000).
Figure 3 A statistical parsimony network for COI sequences of 8 haplotypes (H1-H8) in Gracilaria salicornia.
Fifty-nine haplotypes were identified, and we generated a statistical parsimony haplotype network from these haplotypes, which clustered them into eight chloroplast groups (cpGroup I VIII) (Figs. 4, 5; Additional file 1: Table S1).
We constructed a statistical parsimony network [23] of COI haplotype sequences with TCS version 1.21 [24].
The program implements a statistical parsimony approach using the algorithm described in Templeton et al. [74] to construct haplotype networks.
We examined the relationships of alleles and their geographic segregation by constructing a statistical parsimony network [23] with TCS 1.21 [24].
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