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The reads were then aligned to the mm9 build of the mouse genome using BSmap32.
We aligned these reads to the mm10 build of the mouse genome using TopHat version 2 (ref. 37).
Reads were mapped to the mm9 build of the mouse genome using TopHat (Trapnell et al., 2009).
To test the ability of BAIT to build genomes de novo, we realigned our libraries to the first build of the mouse genome (MGSCv3).
Here, we aligned sequencing data from ChIP-seq and other genomic technologies to a build of the mouse genome containing a single rDNA repeat.
We defined CpG islands using the cpgIslandExt table for the mm9 build of the mouse gene from the UCSC genome browser (Kent et al., 2002).
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We detected six CIGs that are not currently annotated as transcribed regions or genes in the mm9 or mm10 UCSC builds of the mouse genome (Kent et al. 2002).
The remaining five Tsci transcripts had no corresponding annotations in UCSC, Ensembl, or GENCODE builds of the mouse transcriptome (Kent et al. 2002; Harrow et al. 2012; Cunningham et al. 2014).
(Note: The affected exon had been annotated as exon 40 and therefore this mutation was named Δex40 in previous publications. Subsequent builds of the mouse genome identify the exon as exon 41 and hereafter we refer to the mutation as Col4a1Δ ex41).
These sequences were extracted from build rn4 of the rat genome and build mm8 of the mouse genome using annotation from the UCSC genome browser database downloaded on 2007-04-07 [ 61].
These were mapped to build mm9 of the mouse reference genome using v0.12.7 of the Bowtie short-read aligner (Langmead, 2010).
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CEO of Professional Science Editing for Scientists @ prosciediting.com