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Though it is theoretically possible to build a tree using all of those sequences, it is simply computationally too costly to do so for high-throughput processing.
The same run parameters were used to build a tree using parsimony for the combined dataset: the most parsimonious tree consisted of 31,405 steps (see Additional file 12), and a 50% majority rule consensus tree was built from 1000 bootstrap replicates.
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To clarify the phylogenetic situation of the NF-YA genes identified in the transcriptomes, we built a tree using the maximum likelihood method using only the NF-YA domains.
We aligned the TPase-coding segments from 26 Galileo copies and used a ~488-bp region (coordinates: 2699 3131) to build a phylogenetic tree using maximum-likelihood (ML) and Bayesian inference (BI) methods.
As part of the characterization of CsCCD7, CsCCD8a and CsCCD8b, amino acid sequence alignments were carried out, in order to build a phylogenetic tree using the CCD7 and CCD8 protein sequences from a variety of plant species.
Despite important structural shuffling among genomes, even at the species level, we were able to build a phylogenetic tree using rearrangement events between plant mitochondrial genomes that was congruent with a sequence-based tree.
One hundred thirty-seven sequences similar to the Atlantys reverse transcriptase were used to build a phylogenetic tree using the neighbor-joining method.
For example, Bayeux uses Tapestry's[41] unicast routing to build a multicast tree using 4 message types: JOIN, LEAVE, TREE and PRUNE.
Ninety-one randomly picked LTRs from both complete elements and solo-LTRs in indica and japonica O. sativa varieties were used to build a phylogenetic tree using the neighbor-joining method.
One hundred thirteen Atlantys LTR 5′ 300 bp tracts in O. punctata (12), O. officinalis (27), O. minuta (51), and O. alta (23) were used to build a phylogenetic tree using the neighbor-joining method.
These were used to build a phylogenetic tree using maximum likelihood methods (fig. 4).
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