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While visible light cameras, radar, and infrared sensors have been used by various engineering teams to build a physical map around a vehicle, the key component for nearly all autonomous car platforms is LiDAR.
As melon is a species with an average sized genome (445 Mbp), the 5 enzyme/SNaPshot HICF method is the natural choice to build a physical map providing significant coverage of the genome.
To build a physical map, we have fingerprinted about 99,000 BAC clones by the HICF method using an ABI SNaPshot labeling kit and constructed a BAC clone contig map by means of FPC v8.5.3.
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Recently, we have built a physical map with 0.9x genomic coverage using both a BAC library and a genetic map previously developed in our laboratories [ http://melonomics.upv.es/public_files, [ 26]], the first report of such a genomic resource of a Cucurbitaceae species so far.
We then built a physical map of the critical region between these markers using the Zv7 genomic assembly to obtain contigs, and bridged the gaps between contigs using BAC information from the Zebrafish Genome Fingerprinting Project and sequences from the Ensembl trace repository.
We developed 79 STS (Sequence Tagged Site) markers to build a physical RH (radiation hybrid) map and 34 genetic markers to extend the genetic map of GGA16.
The company is also starting to build a physical presence.
This indicated that the default FPC parameters and/or data filtering were not stringent enough to build a robust physical map for this more complex dataset.
The deletion in 39B3 was further characterized by PCR on available Citrus BACs, which helped us to build a partial physical map of the deletion.
The above results indicated that the microsynteny between Citrus and Populus genomes was high enough to predict gene arrangement and to build a partial physical map of a Citrus genomic segment of about 700 kb, as inferred from the length of poplar homologous regions.
A physical map was build using HIFC technique [ 8] in Fingerprinted Contigs software (FPC V9.3; [ 11]) following "physical map assembly guideline" established by TriticeaeGenome project and accepted by the International Wheat Genome Sequencing Consortium IWGSCC).
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