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We demonstrate that a useful proportion of zebra finch EST-SSRs are likely to be polymorphic, and that they can be used to build a linkage map.
Recently, a similar conclusion was reached in a paper that combined SNP, microsatellite and AFLP data to build a linkage map for the chicken genome [ 23].
To this end, we first developed new SNP markers specific to brook charr in coding gene regions and used them in combination with previously published microsatellite markers to build a linkage map.
As the first application to genetic mapping in fishes, the so-called "RAD-sequencing" approach has been used to build a linkage map comprising 8400 SNP markers in the longnose gar (Lepisosteus osseus) (Amores et al. 2011).
> -wrap-foot> Wild soybeans including JP-36121 are thought to be more genetically diverse than cultivated soybeans, with the result that the genome panel was able effectively to build a linkage map of the JI population.
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To build a consensus linkage map for the Pacific oyster, we used individual family ML framework maps, with the exceptions described in Materials and Methods.
We then re-constructed component maps from seven quality-filtered datasets that had sufficient numbers of lines and markers to build a reliable linkage map.
They have been used to build a genetic linkage map that can be applied to quantitative trait locus (QTL) discovery.
The JoinMap 2.0 package [ 32] was used to build a genetic linkage map for each individual family and for the combined genotype data from all families.
The JoinMap 2.0 package (Stam and Van Ooijen, 1995) was used to build a genetic linkage map for each of the two families using the same parameters as described previously (Wondji et al., 2007b).
We used the OWB population as a mapping resource in order to directly compare RAD and EST-based SNP maps and to assess the quality and utility of a linkage map built with the two types of data.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com