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These buffers were tested in different molarities for their capacity to elute rbST from the monolith micro-columns.
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Antibody samples serially diluted in running buffer were tested in duplicate with the final data sets collated from two separate experiments.
The efficiency of iron removal from Lf in citrate buffer was tested in a pH range of 2 5 (Fig. 4).
First, various buffers were tested as shown in Figure 4 (A).
However, all buffers used were tested in a control group of chicken embryos (PBS control) in each individual experiment.
Two treatments (with and without a cornstalk buffer strip) were tested in the following four runs: 1) without cornstalk buffer strip, 2) with cornstalk buffer strip in the third rain event, 3) with cornstalk buffer strip in the second rain event, 4) with continuous cornstalk buffer strip in all three successive rainfall events.
Therefore, Sorenson's phosphate buffers from pH 6.0 to 8.0 were tested in steps of 0.5 pH units.
The native PHS and PHS nanogel stabilizing against thermal denaturation were tested in acetate buffer (100 mM, pH 4.5) at 60 °C and the activity was determined after sampling periodically as described above.
The cargo-loaded vesicles were tested in PBS buffer with different glucose concentrations, including a typical hyperglycemic level (400 mg/dL) and a normal level (100 mg/dL).
All measures were tested in a calcium buffer containing 0,15 μM of free Ca2+.
Fluorescence characteristics of the synthesised β-diketones with Eu were tested in a glycine buffer at pH 2.3 containing various concentrations of the water protecting agent TOPO (25 120 μM), detergent Triton X-100 (0.05–0.2% v/ v) and the β-diketone (1 100 μM) to be tested.
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