Primer pairs of COX-2, ED1, ubiquitin C (UBC), protector RNAse inhibitor, and 1x RT buffer were supplied by Invitrogen (Darmstadt, Germany).
Enhanced chemiluminescence (ECL) western blotting substrate and co-IP kit and RIPA lysis buffer were supplied by Thermo (Fremont, CA, USA).
Pure BoNT-A (vials of 100 μg, 150 kD) from Clostridium botulinum, lyophilized powder containing 20 mM HEPES and 1.25% lactose when reconstituted with 100 μL buffer, was supplied by Sigma-Aldrich (Zwijndrecht, The Netherlands).
All other solvents (synthesis grade) as well as NaCl and buffer salts were supplied by Carl Roth (www.roth.de).
All the salts necessary to prepare phosphate buffer saline (PBS) were supplied by Sigma-Aldrich.
Phosphate-buffered solution (PBS) Dulbecco and trypsin-EDTA (0.05% trypsin, 0.53 m M EDTA.4Na in Hanks-buffered saline solution) were supplied by Gibco (Invitrogen Corporation).
Cell culture reagents, fetal bovine serum (FBS), Dulbecco's modified eagle medium (DMEM, high glucose), penicillin-streptomycin, trypsin/EDTA, and Dulbecco's phosphate buffer saline (PBS) were supplied by Gibco BRL (Carlsbad, CA), and the SK-BR3 cells (breast cancer cells) were purchased from Korean Cell Line Bank.
Dulbecco's Modified Eagle's medium (DMEM), Roswell Park Memorial Institute Medium RPMI-16400) and phosphate buffer saline (PBS) tablet were supplied by Lonza.
Hanks Balanced Salt Solution (HBSS), phosphate buffered saline (PBS) and fibronectin were supplied by Sigma-Aldrich.
All other solvents (synthesis grade) as well as sodium chloride and the buffer salts CHES, MES, and CAPS were supplied by Carl Roth (www.roth.de).
