Sentence examples for buffers were prepared in from inspiring English sources

All purification and dialysis buffers were prepared in sterile endotoxin-free water.

The assay buffers were prepared in KCl-HCl buffers (pH 1.1 2.2) and sodium citrate acid buffers (pH 2.2 8.0).

All buffers were prepared in deionized water; filtered through 0.2μm membrane, and degassed for 15 min. Column and all buffers were equilibrated to room temperature before use.

CPB buffers were prepared in the range of pH 4.0 7.5 and the reactions were performed at 30, 37, 45, 55 and 65°C.

Unless otherwise noted, all samples and buffers were prepared in deionized water prepared using a Milli-Q water purification system (Millipore Corp., Bedford, MA, USA).

In our case, the assay buffers were prepared in 20 L lots with an initial Ca2+ concentration of 200 μM and required 361.6 mL of 10 mM EGTA to titrate the free Ca2+ concentration to the desired concentration of 300 nM.

Sensor solutions in assay buffer were prepared in bulk in buffer containing 50 mM Tris, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT, 0.01% Brij-35P, and 1 mM ATP (final assay concentrations) and aliquoted across 96-well plates.

To determine the LC99.9 values of LL-37 and BP2, 25-μl aliquots of two-fold serially diluted peptide in incubation buffer were prepared in polypropylene microtiter plates (Costar Corning) and to each of the wells, 25 μl of a bacterial suspension containing 2 × 10 CFU/ml was added.

Assay buffer was prepared in either H2O or D2O.

The calcium-free buffer was prepared in the same manner while CaCl2 was not included.

A total of 50 mM ammonium formate buffer was prepared in water and its pH adjusted to 3.0 with formic acid.