Sentence examples for buffers were prepared by from inspiring English sources
The inocula and the necessary media and buffers were prepared by following a study-specific procedure.
In assays examining PLC Ca2+ sensitivity, Ca2+ buffers were prepared by EGTA/CaCl2 admixture, as previously described elsewhere (8, 13).
Running buffers were prepared by dissolving the appropriate amount of sodium phosphate in Milli-Q water, adjusting the pH with 1 M NaOH before completing the volume to get the desired buffer concentration (25 mM).
For Ca2+ titration, Ca2+/EDTA, Ca2+/HEDTA [ N- 2-hydroxyethyl)ethyleN- 2-hydroxyethyl N- 2-hydroxyethyl], and Ca2+/NTA buffethylenediamineed by mixing Ca2+-saturated aNd Ca2+-free buffers (30 mM Mops, 100 mM KCl aN′ 10 mM chelating reagent, pH 7.2, either with or without 10 mM Ca2+) to achieve buffer-free Ca2+ concentrations from 0 to 1.13 mM.
Salt buffers were prepared by dissolving a known quantity of the acid in water and titrating with the base to attain the desired pH (example: dissolving high-performace liquid chromatography (HPLC -grade HPLC -gradeter and titrating witH3PO4 or trinthylamine).
Charge-buffers were prepared by shaking such ligand-loaded beads in buffer A containing appropriate concentration of protein at 37°C for 15 min with a frequency of 85 strokes per min and amplitude 3 cm.
The electrolyte solutions of NaCl and borate buffer were prepared by dissolving NaCl, and NaCl and borate acid (i.e., NaCl+H3BO3) in deionized water, respectively.
Similarly, in co-cultivation experiments growth curves for the three NG in the presence of each of the LB, with and without MES buffer, were prepared by plotting median values.
TBS buffer was prepared by mixing 150 mM NaCl, 50 mM Tris and 5 mM NaHCO3 followed by stirring over chelex overnight and subsequent filtration.
Tris buffer was prepared by mixing 100 ml 0.1 M tris(hydroxymethyl)aminomethane with 29.4 ml of 0.1 M HCl [13].
